Khodeir, Salwa S. and Elkerdany, Tahany A. and Saad, Abeer Attia and Sakhawy, Yasmin N. El and Hamed, Gehan M. and Hassan, Noha Bassiouny (2018) Role of Immunostaining in Detecting Extra-Pattern and Subtle Lymphomatous Infiltration in Bone Marrow Biopsies of NHL Patients. Open Journal of Blood Diseases, 08 (02). pp. 27-36. ISSN 2164-3180
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Abstract
Introduction: Immunohistochemistry (IHC) enables the examination of a greater number of trephine biopsy levels and is helpful in determining additional scattered malignant cells. The aim of this study is to detect extra-pattern and subtle lymphomatous infiltration in bone marrow biopsies using CD20 and CD3 immunostaining. Patients and Methods: This study was conducted on 100 newly diagnosed Non Hodgkin Lymphoma (NHL) patients. Their bone marrow trephine biopsies were assessed on routine histology [Hematoxylin and Eosin (H & E)], and were further subjected to IHC using CD20 and CD3. Results: Pattern of involvement by H & E was highlighted by IHC. It showed additional interstitial pattern in 9 cases, parasinusoidal streaks in one case and highlighted a patchy pattern in another case with interstitial involvement on H & E. IHC also detected subtle infiltrations on additional 5.5% cases compared with histology alone. It helped in differentiating reactive (12 cases) and malignant lymphoid infiltration (33 cases). Conclusion: CD20 and CD3 immunostaining performed routinely on bone marrow trephine biopsies has the ability to reveal extra-pattern of infiltration and improve detection of subtle lymphoid involvement. A combined procedure identifying several distinctive features, in particular histotopography and IHC, provides a promising way of discriminating reactive from neoplastic lymphoid infiltrates in bone marrow trephine biopsies.
Item Type: | Article |
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Subjects: | ScienceOpen Library > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 06 Apr 2023 05:18 |
Last Modified: | 07 Sep 2024 10:19 |
URI: | http://scholar.researcherseuropeans.com/id/eprint/928 |