Enhanced bio-catalytic and tolerance properties of an indigenous cellulase through xerogel immobilization

Iqbal, Hafiz Muhammad Nasir and Kamal, Shagufta and Ahmed, Ishtiaq and Naveed, Muhammad Tahir (2012) Enhanced bio-catalytic and tolerance properties of an indigenous cellulase through xerogel immobilization. Advances in Bioscience and Biotechnology, 03 (04). pp. 308-313. ISSN 2156-8456

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Abstract

Today, demand exists for cost-effective production of industrially important enzymes from entire scientific sectors. By keeping in mind the extensive industrial applications of cellulase, this study was performed to immobilize the indigenous enzyme produced from Trichoderma viride under pre-optimized SSF of an agricultural waste material, wheat straw. To enhance the bio-catalytic and tolerance properties of the present enzyme gel matrix immobilization engineering was applied. Previously, 2.33~fold purified novel cellulase was immobilized in to a xerogel matrix of TMOS and PTMS. FTIR spectroscopy confirmed the successful immobilization of cellulase. The free and immobilized cellulase was characterized and stability profile showed that after 24 h incubation, immobilization enhanced the thermo-stability up to 75% against 80℃ as compare to the free enzyme. Xerogel matrix immobilization enhanced the catalytic efficiency of entrapped enzyme than that of the free cellulase. Among activators/inhibitors SDS, EDTA, and Hg2+ showed inhibitory effect while, gel matrix immobilization enhanced 80% tolerance capacity of the cellulase against inactivating agents.

Item Type: Article
Subjects: ScienceOpen Library > Biological Science
Depositing User: Managing Editor
Date Deposited: 22 Mar 2023 05:58
Last Modified: 13 Sep 2024 07:28
URI: http://scholar.researcherseuropeans.com/id/eprint/812

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