MORPHOGISTOSCHEMICAL CHARACTERISTIC OF THE IMPLANT FROM POLYLACTIC ACID

Aim. The study was conducted for the characteristics of the morphoimmunohistochemical status of the implant from polylactic acid (PLA) located in the dermis.

Materials and methods. The study was performed on rats (30 individuals) with subdermal administration of the drug in a volume of 0,05 ml. The results were evaluated after two weeks, 1 and 2 months after the injection. The sections were stained with hematoxylin and eosin, according to Van Gieson, with the help of Mallory and Masson reaction. Collagen fibers were studied after the staining with picrosirius red under polarized light. Monoclonal antibodies of CD 68 and antibodies to vimentin were used to determine the expression of immunohistochemical markers.

Results. It is shown that the rate of collagen synthesis varies in different parts of the dermis. There is a sharp increase in the volume of the fibrous component around the implant, and there is only a tendency to increase in the synthesis in the implant itself. In this case, the increase in the volume of the extracellular matrix is associated with type I and III collagen.

Conclusion. The detected activation of collagen synthesis by fibroblasts can be used as a regulator of the volume of the matrix, which is very important for the modern regenerative medicine.