Expression of Epsilon Toxin from Clostridium perfringens Type D in E. coli Rosetta (DE3): Study on a New Vaccine Production

Clostridium perfringens is a Gram-positive anaerobic bacterium which is divided based on major toxins into five types (A-E). C. perfringens type D causes fatal enterotoxaemia (pulpy kidney) in sheep and goats that causes heavy economic losses in domestic animals. However current enterotoxaemia vaccine has been effective in controlling of disease; Strategies for the development of effective vaccine can be achieved by the production of new generation experimental vaccines. The aim of this study was cloning and expression of epsilon toxin of C. perfringens type D in E. coli Rosetta (DE3) to improve of the immunity. Epsilon toxin gene was cloned into pJET1.2/blunt vector and pET22b (+) expression vector and finally transformed into E. coli Rosetta competent cells (DE3). The result showed that the epsilon gene of C. perfringens type D can be cloned and expressed in E. coli RosettaTM (DE3) successfully. E. coli was suitable host for the expression of C. perfringens epsilon toxin. Clearly, future research is open for further refinement of the study.