Evteev, V. A. and Kazakov, R. E. and Prokof'ev, A. B. and Mazerkina, I. A. and Bunyatyan, N. D. (2018) Activity of renal organic anion transporters in a model of ischemia and reperfusion injury in vitro. Sechenov Medical Journal (4). pp. 25-27. ISSN 2218-7332
78-66-1-PB.pdf - Published Version
Download (165kB)
Abstract
The aim of the work is to study the functional characteristics of SLC transporters of organic anions: OAT1 and OAT3 in normal conditions and in model ischemia/reperfusion injury.Materials and methods. The HEK293 cell line was used as a model for the study. Conditions of ischemia/reperfusion injury were created by the previously described method. The activity of the transporters was assessed by the capture of the marker substrate - fluorescein. The concentration of fluorescein was measured using a plate fluorimeter. The results were normalized by the amount of total protein.Results. In condition of ischemia/reperfusion injury, the activity of organic anion transporters decreased in comparison with the norm. The data obtained allow us to conclude that in conditions of ischemia/reperfusion injury, the concentration of dicarboxylic acids in the cell is low, which in turn can lead to a decrease activity of transporters.
Item Type: | Article |
---|---|
Subjects: | ScienceOpen Library > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 18 Feb 2023 12:25 |
Last Modified: | 24 Aug 2024 12:56 |
URI: | http://scholar.researcherseuropeans.com/id/eprint/565 |