Routy, Bertrand and Richard, Corentin and Benlaïfaoui, Myriam and Lapierre, Simon Grandjean and Armstrong, Nicholas and Al-Saleh, Afnan and Boko, Mélodie and Jacq, Maxime and Watson, Ian R. and Mihalcioiu, Catalin and Elkrief, Arielle and Tidjani Alou, Maryam and Messaoudene, Meriem and Diop, Khoudia (2022) Characterization of Alistipes montrealensis sp. nov., Isolated from Human Feces of a Patient with Metastatic Melanoma Treated with Immune Checkpoint Inhibitors. Microbiology Research, 13 (1). pp. 140-151. ISSN 2036-7481
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Abstract
Fecal microbiome culturomics of a cancer patient treated with immune checkpoint inhibitors led to the identification of a Gram-negative, rod-shaped, obligate anaerobic, non-motile, non-spore-forming bacterium, designated strain kh20T, which was phylogenetically assigned to the genus Alistipes. Strain kh20T demonstrated a 98.61% 16S rRNA sequence similarity with A. shahii WAL 8301T. The bacteria cells generated catalase but no oxidase. Iso-C15:0 (26.6%), anteiso-C15:0 (19.9%), and iso-C17:0 (17.2%) were the major cellular fatty acids identified in its composition. The G+C content of its genome was 57.2%. Strain kh20T showed significantly low values for DNA-DNA Hybridation (DDH ≤ 33.70%) and Average Nucleotide Identity (ANI ≤ 86.35%) compared with other Alistipes species. Based on these findings, we concluded that strain kh20T represented a novel bacterium, and we proposed the name Alistipes montrealensis sp. nov. (CECT 30384 and CSUR Q6005).
Item Type: | Article |
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Subjects: | ScienceOpen Library > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 15 Jun 2023 05:24 |
Last Modified: | 26 Oct 2024 04:04 |
URI: | http://scholar.researcherseuropeans.com/id/eprint/1556 |