Determination of Vitamin B6, B9 and B12 in Halophytes by Solid Phase Extraction Followed by HPLC-UV

Chamkouri, Narges and Vafaeizadeh, Mona and Mojaddami, Ayyub and Hosseini, Seyyed and Isfahani, Tahereh and Golkhajeh, Aida and Lariche, Milad and Malaekeh, Seyed (2018) Determination of Vitamin B6, B9 and B12 in Halophytes by Solid Phase Extraction Followed by HPLC-UV. Journal of Pharmaceutical Research International, 20 (4). pp. 1-6. ISSN 24569119

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Abstract

In this work, solid phase extraction (SPE) was proposed for simultaneous determination of trace levels of vitamin B6, B9 and B12. High performance liquid chromatography with UV detection (HPLC-UV) was used. The experimental conditions including pH of sample and type and volume of extraction solvent were studied by one-factor-at-a time method. The limit of detection in the optimal conditions was 14.91, 10.03, and 7.25 ngkg-1 for vitamins B6, B9 and B12 respectively and relative standard deviations (RSD) of the analysis less than 8% (n= 3). Recoveries of vitamins B6, B9 and B12 were in the ranges of 80–93%. The proposed method was successfully applied for the simultaneous determination of vitamin B6, B9 and B12 in halophyte samples such as, Salicornia, Suaeda monoica, Suaeda aegyptiaca, Sueada vera and Sueada martima.

Item Type: Article
Subjects: ScienceOpen Library > Medical Science
Depositing User: Managing Editor
Date Deposited: 18 May 2023 05:43
Last Modified: 23 Sep 2024 04:17
URI: http://scholar.researcherseuropeans.com/id/eprint/1280

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