Performance of Shiitake Mushrooms (Lentinula edodes) on Some Selected Agro-Based Waste Extracts Media under In vitro Conditions

Shiitake mushrooms (Lentinula edodes), known for growing on decaying deciduous trees, are particularly notable for their health benefits. In Telangana state, the climate and agricultural waste provide optimal conditions for mushroom cultivation, which can boost the local economy and nutrition. The present investigation was taken up in Completely randomized block design (CRBD) with 3 replications to evaluate the performance of shiitake mushroom (Lentinula edodes) on seventeen different agro-based waste extract media and maintained at two different temperatures viz., 20°C and 24°C under in vitro conditions. Results revealed that at 20°C, the maximum mycelium growth of L. edodes was recorded in treatment T 17 (PDA) (75.33 mm, 90.00 mm) on 7 DAI (Days After Inoculation) and 14 DAI respectively, while minimum mycelium growth was recorded in T 8 (Sawdust + Paddy Straw) (22.00 mm, 35.00 mm). Similarly at 24°C treatment T 17 (PDA) and T 12 (Eucalyptus Bark + Wheat Grains and Sorghum Grains) recorded the maximum mycelium growth (90.00 mm, 90.00 mm) and T 8 (Sawdust + Paddy Straw) recorded minimum mycelium growth (30.33 mm, 42.97 mm) on 7 DAI and 14 DAI respectively. The Principal Component Analysis of recorded values of the mycelial growth was shown in the form of eigen values, the highest eigen values were recorded for L. edodes mycelial growth on 7 DAI and 14 DAI at 24°C indicating the significant influence of temperature and media.