Ginger and Honeybee Modulates MTX-induced Oxidative Stress in Kidney of Rats

Aim: The purpose of this study was to examine the curative effects of aqueous extract of ginger and honey bee solutions on methotrexate (MTX) induced kidney toxicity.

Materials and Methods: Twenty-eight adult female Wistar albino rats (aged 8–12 weeks). It is divided into four groups (n = 7): group I used as control negative; 2 methotrexate groups: G2, G3 and G4 injected with vehicle intraperitoneally of MTX (20 mg/kg body weight) one dose only. In treating groups 3&4 rats were pre-treated and continue seven days with aqueous extract of ginger and the honeybee solution, respectively.

Results and Discussion: It had indicated the treated rats with aqueous extracts of ginger and\or honey bee solution had caused the lowest significantly decreased (P ≤ 0.05) at urea concentration in blood. As well as significantly increased at (P ≤ 0.05) in Hb, RBC,S and WBc.s markers compared to the control +ve. In addition, treatment rats with aqueous extracts of ginger and\or honey bee solution had caused significant increase in total antioxidant levels (1.40±0.22 & 1. 70±0.24 mM / L), respectively, compared the rats give MTX only 1.00±0.96 mM/L. Concerning, the MDA concentration in the treatment groups with aqueous extract of ginger or honey bee solution the data indicated that improvement and significantly decreased in the MDA levels (P< 0.05) compared to the control +ve. The histopathology results supported this conclusion.

Recommendation: Ginger and honey bee solution probably protect from MTX induced kidney damage by scavenging of free radicals and inflammation.